Publications

 *, **: equality contributed, ^✝: corresponding author

Original article

1. Irie N*, Lee SM*, Lorenzi V, Xu H, Chen J, Inoue M, Kobayashi T, Sancho-Serra C, Drousioti E, Dietmann S, Vento-Tormo R, Song CX, Surani MA^✝.
   DMRT1 regulates human germline commitment.
   Nat Cell Biol. 2023 Sep 14. doi: 10.1038/s41556-023-01224-7.
2. Iwatsuki K, Oikawa M, Kobayashi H, Penfold CA, Sanbo M, Yamamoto T, Hochi S, Kurimoto K, Hirabayashi M, Kobayashi T^✝.
   Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells.
   Cell Rep Methods. 2023 Jul 27;3(8):100542. doi: 10.1016/j.crmeth.2023.100542. eCollection 2023 Aug 28.
   [Press release https://www.ims.u-tokyo.ac.jp/imsut/jp/about/press/page_00256.html]
   [Preview https://www.sciencedirect.com/science/article/pii/S2667237523002199]
3. Castillo-Venzor A, Penfold CA, Morgan MD, Tang WW, Kobayashi T, Wong FC, Bergmann S, Slatery E, Boroviak TE, Marioni JC, Surani MA.
   Origin and segregation of the human germline.
   Life Sci Alliance. 2023 May 22;6(8):e202201706. doi: 10.26508/lsa.202201706. Print 2023 Aug.
4. Richard Albert J, Kobayashi T, Inoue A, Monteagudo-Sánchez A, Kumamoto S, Takashima T, Miura A, Oikawa M, Miura F, Takada S, Hirabayashi M, Korthauer K, Kurimoto K, Greenberg MVC, Lorincz M, Kobayashi H^✝.
   Conservation and divergence of canonical and non-canonical imprinting in murids.
   Genome Biol. 2023 Mar 14;24(1):48. doi: 10.1186/s13059-023-02869-1.
5. Tang WWC*, Castillo-Venzor A*, Gruhn WH*, Kobayashi T, Penfold CA, Morgan MD, Sun D, Irie N, Surani MA^✝.
   Sequential enhancer state remodelling defines human germline competence and specification
   Nat Cell Biol. 2022 Apr;24(4):448-460. doi: 10.1038/s41556-022-00878-z. Epub 2022 Apr 11.
6. Oikawa M*, Kobayashi H, Sanbo M, Mizuno N, Iwatsuki K, Takashima T, Yamauchi K, Yoshida F, Yamamoto T, Shinohara T, Nakauchi H, Kurimoto K, Hirabayashi M^✝, Kobayashi T*^✝.　
   Functional primordial germ cell-like cells from pluripotent stem cells in rats.
   Science. 2022 Apr 8;376(6589):176-179. doi: 10.1126/science.abl4412. Epub 2022 Apr
   [Press release https://www.ims.u-tokyo.ac.jp/imsut/jp/about/press/page_00164.html]
7. Oikawa M*, Nagae M*, Mizuno N, Iwatsuki K, Yoshida F, Inoue N, Uenoyama Y, Tsukamura H, Nakauchi H, Hirabayashi^✝ M, Kobayashi T^✝.
   Generation of Tfap2c-T2A-tdTomato knock-in reporter rats via adeno-associated virus-mediated efficient gene targeting.
   Mol Reprod Dev. 2022 Mar;89(3):129-132. doi: 10.1002/mrd.23562. Epub 2022 Feb 15.
8. Kinoshita M, Kobayashi T, Planells B, Klisch D, Spindlow D, Masaki H, Bornelöv S, Stirparo GG, Matsunari H, Uchikura A, Lamas-Toranzo I, Nichols J, Nakauchi H, Nagashima H, Alberio R^✝, Smith A^✝.
   Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species.
   Development. 2021 Dec 1;148(23):dev199901. doi: 10.1242/dev.199901. Epub 2021 Dec 7.
9. Kobayashi T*^✝, Castillo-Venzor A*, Penfold CA, Morgan M, Mizuno N, Tang WWC, Osada Y, Hirao M, Yoshida F, Sato H, Nakauchi H, Hirabayashi M, Surani MA^✝.
   Tracing the emergence of primordial germ cells from bilaminar disc rabbit embryos and pluripotent stem cells
   Cell Rep. 2021 Oct 12;37(2):109812. doi: 10.1016/j.celrep.2021.109812.
   [Press release https://www.ims.u-tokyo.ac.jp/imsut/jp/about/press/page_00127.html]
10. Kobayashi T*^✝, Goto T*, Oikawa M*, Sanbo M, Yoshida F, Terada R, Niizeki N, Kajitani N,  Kazuki K, Kazuki Y, Hochi S, Nakauchi H, Surani MA, Hirabayashi M^✝.
    Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats.
    Nat Commun. 2021 Feb 26;12(1):1328. doi: 10.1038/s41467-021-21557-x.
    [Press release https://www.nips.ac.jp/release/2021/02/post_430.html]
11. Kobayashi T*, Kobayashi H*, Goto T, Takashima T, Oikawa M, Ikeda H, Terada R, Yoshida F, Sanbo M, Nakauchi H, Kurimoto K, Hirabayashi M^✝.
    Germline Development in Rat Revealed by Visualization and Deletion of Prdm14.
    Development. 2020 Feb 21;147(4):dev183798. doi: 10.1242/dev.183798.
12. Gao X*, Nowak-Imialek M*, Chen X*, Chen D, Herrmann D, Ruan D, Chen ACH, Eckersley-Maslin MA, Ahmad S, Lee YL, Kobayashi T, Ryan D, Zhong J, Zhu J, Wu J, Lan G, Petkov S, Yang J, Antunes L, Campos LS, Fu B, Wang S, Yong Y, Wang X, Xue SG, Ge L, Liu Z, Huang Y, Nie T, Li P, Wu D, Pei D, Zhang Y, Lu L, Yang F, Kimber SJ, Reik W, Zou X, Shang Z, Lai L, Surani A, Tam PPL, Ahmed A, Yeung WSB, Teichmann SA, Niemann H^✝, Liu P^✝.
    Establishment of porcine and human expanded potential stem cells.
    Nat Cell Biol. 2019 Jun;21(6):687-699. doi: 10.1038/s41556-019-0333-2. Epub 2019 Jun 3.
13. Goto T, Hara H, Sanbo M, Masaki H, Sato H, Yamaguchi T, Hochi S, Kobayashi T, Nakauchi H, Hirabayashi M^✝.
    Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats.
    Nat Commun. 2019 Feb 5;10(1):451. doi: 10.1038/s41467-019-08394-9.
14. Oldani G, Peloso A, Vijgen S, Wilson EM, Slits F, Gex Q, Morel P, Delaune V, Orci LA, Yamaguchi T, Kobayashi T, Rubbia-Brandt L, Nakauchi H, Lacotte S, Toso C^✝.
    Chimeric liver transplantation reveals interspecific graft remodelling.
    J Hepatol. 2018 Nov;69(5):1025-1036. doi: 10.1016/j.jhep.2018.07.008. Epub 2018 Jul 20.
15. Cheetham SW*, Gruhn WH*, van den Ameele J, Krautz R, Southall TD, Kobayashi T, Surani MA, Brand AH^✝.
    Targeted DamID reveals differential binding of mammalian pluripotency factors.
    Development. 2018 Oct 17;145(20). pii: dev170209. doi: 10.1242/dev.170209.
16. Yamaguchi T*, Sato H*, Kobayashi T, Kato-Itoh M, Goto T, Hara H, Mizuno N, Yanagida A, Umino A, Hamanaka S, Suchy F, Masaki H, Ota Y, Hirabayashi M, Nakauchi H^✝.
    An Interspecies Barrier to Tetraploid Complementation and Chimera Formation.
    Sci Rep. 2018 Oct 16;8(1):15289. doi: 10.1038/s41598-018-33690-7.
17. Hackett JA*, Huang Y*, Günesdogan U*, Gretarsson KA, Kobayashi T, Surani MA^✝.
    Tracing the transitions from pluripotency to germ cell fate with CRISPR screening.
    Nat Commun. 2018 Oct 16;9(1):4292. doi: 10.1038/s41467-018-06230-0.
18. Hamanaka S, Umino A, Sato H, Hayama T, Yanagida A, Mizuno N, Kobayashi T, Kasai M, Suchy FP, Yamazaki S, Masaki H, Yamaguchi T, Nakauchi H^✝.
    Generation of Vascular Endothelial Cells and Hematopoietic Cells by Blastocyst Complementation.
    Stem Cell Reports. 2018 Oct 9;11(4):988-997. doi: 10.1016/j.stemcr.2018.08.015. Epub 2018 Sep 20.
19. Bao S^✝, Tang WW, Wu B, Kim S, Li J, Li L, Kobayashi T, Lee C, Chen Y, Wei M, Li S, Dietmann S, Tang F, Li X, Surani MA^✝.
    Derivation of hypermethylated pluripotent embryonic stem cells with high potency.
    Cell Res. 2018 Jan;28(1):22-34. doi: 10.1038/cr.2017.134. Epub 2017 Oct 27.
20. Kobayashi T*, Zhang H*, Tang WWC, Irie N, Withey S, Klisch D, Sybirna A, Dietmann S, Contreras DA, Webb R, Allegrucci C, Alberio R^✝, Surani MA^✝.
    Principles of early human development and germ cell program from conserved model systems.
    Nature. 2017 Jun 15;546(7658):416-420. doi: 10.1038/nature22812. Epub 2017 Jun 7.
21. Hackett JA*, Kobayashi T*, Dietmann S, Surani MA^✝
    Activation of lineage-regulators and transposable elements across a pluripotent spectrum.
    Stem Cell Reports. 2017 Jun 6;8(6):1645-1658. doi: 10.1016/j.stemcr.2017.05.014.
22. Yamaguchi T*, Sato H*, Kato-Itoh M, Goto T, Hara H, Sanbo M, Mizuno N, Kobayashi T, Yanagida A, Umino A, Ota Y, Hamanaka S, Masaki H, Rashid ST, Hirabayashi M, Nakauchi H^✝.
    Interspecies organogenesis generates autologous functional islets.
    Nature. 2017 Feb 9;542(7640):191-196. doi: 10.1038/nature21070. Epub 2017 Jan 25.
23. Tarusawa E, Sanbo M, Okayama A, Miyashita T, Kitsukawa T, Hirayama T, Hirabayashi T, Hasegawa S, Kaneko R, Toyoda S, Kobayashi T, Kato-Itoh M, Nakauchi H, Hirabayashi M, Yagi T, Yoshimura Y^✝.
    Establishment of high reciprocal connectivity between clonal cortical neurons is regulated by the Dnmt3b DNA methyltransferase and clustered protocadherins.
    BMC Biol. 2016 Dec 2;14(1):103. doi: 10.1186/s12915-016-0326-6.
24. Hara H, Goto T, Takizawa A, Sanbo M, Jacob HJ, Kobayashi T, Nakauchi H, Hochi S, Hirabayashi M^✝.
    Rat Blastocysts from Nuclear Injection and Time-Lagged Enucleation and Their Commitment to Embryonic Stem Cells.
    Cell Reprogram. 2016 Apr;18(2):108-15. doi: 10.1089/cell.2015.0084.
25. Murakami K*, Günesdogan U*, Zylicz JJ, Tang WW, Sengupta R, Kobayashi T, Kim S, Butler R, Dietmann S, Surani MA^✝.
    NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.
    Nature. 2016 Jan 21;529(7586):403-7. doi: 10.1038/nature16480. Epub 2016 Jan 11.
26. Goto T*, Kobayashi T*, Hara H, Sanbo M, Hochi S, Nakauchi H, Hirabayashi M^✝.
    Knock-in of a histone H2B-tdTomato reporter into the Rosa26 locus allows visualization of cell nuclei in rats.
    Mol Reprod Dev. 2015 Dec;82(12):916-7. doi: 10.1002/mrd.22584. Epub 2015 Sep 21.
27. Yanagida A, Chikada H, Ito K, Umino A, Kato-Itoh M, Yamazaki Y, Sato H, Kobayashi T, Yamaguchi T, Nakayama KI, Nakauchi H^✝, Kamiya A^✝.
    Liver maturation deficiency in p57Kip2-/- mice occurs in a hepatocytic p57Kip2 expression-independent manner.
    Dev Biol. 2015 Nov 15;407(2):331-43. doi: 10.1016/j.ydbio.2015.07.004. Epub 2015 Jul 9.
28. Masaki H, Kato-Itoh M, Umino A, Sato H, Hamanaka S, Kobayashi T, Yamaguchi T, Nishimura K, Ohtaka M, Nakanishi M, Nakauchi H^✝.
    Interspecific in vitro assay for the chimera-forming ability of human pluripotent stem cells.
    Development. 2015 Sep 15;142(18):3222-30. doi: 10.1242/dev.124016. Epub 2015 May 28.
29. Irie N*, Weinberger L*, Tang WW*, Kobayashi T, Viukov S, Manor YS, Dietmann S, Hanna JH^✝, Surani MA^✝.
    SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate.
    Cell. 2015 Jan 15;160(1-2):253-68. doi: 10.1016/j.cell.2014.12.013. Epub 2014 Dec 24.
30. Kobayashi T, Kato-Itoh M, Nakauchi H^✝.
    Targeted Organ Generation Using Mixl1-Inducible Mouse Pluripotent Stem Cells in Blastocyst Complementation.
    Stem Cells Dev. 2015 Jan 15;24(2):182-9. doi: 10.1089/scd.2014.0270.
31. Hayama T, Yamaguchi T, Kato-Itoh M, Hamanaka S, Kawarai M, Sanbo M, Tamura C, Lee YS, Yanagida A, Murayama H, Mizuno N, Umino A, Sato H, Yamazaki S, Masaki H, Kobayashi T, Hirabayashi M, Nakauchi H^✝.
    Generation of mouse functional oocytes in rat by xeno-ectopic transplantation of primordial germ cells.
    Biol Reprod. 2014 Oct;91(4):89. doi: 10.1095/biolreprod.114.121640. Epub 2014 Aug 27.
32. Ito K, Yamazaki S, Yamamoto R, Tajima Y, Yanagida A, Kobayashi T, Kato-Itoh M, Kakuta S, Iwakura Y, Nakauchi H^✝, Kamiya A^✝.
    Gene Targeting Study Reveals Unexpected Expression of Brain-expressed X-linked 2 in Endocrine and Tissue Stem/Progenitor Cells in Mice.
    J Biol Chem. 2014 Oct 24;289(43):29892-911. doi: 10.1074/jbc.M114.580084. Epub 2014 Aug 20.
33. Matsunari H, Kobayashi T, Watanabe M, Umeyama K, Nakano K, Kanai T, Matsuda T, Nagaya M, Hara M, Nakauchi H, Nagashima H^✝.
    Transgenic Pigs with Pancreas-specific Expression of Green Fluorescent Protein.
    J Reprod Dev. 2014 Jul 3;60(3):230-7. doi: 10.1262/jrd.2014-006. Epub 2014 Apr 21.
34. Toyoda S, Kawaguchi M, Kobayashi T, Tarusawa E, Toyama T, Okano M, Oda M, Nakauchi H, Yoshimura Y, Sanbo M, Hirabayashi M, Hirayama T, Hirabayashi T, Yagi T^✝.
    Developmental epigenetic modification regulates stochastic expression of clustered protocadherin genes, generating single neuron diversity.
    Neuron. 2014 Apr 2;82(1):94-108. doi: 10.1016/j.neuron.2014.02.005.
35. Hirabayashi M, Goto T, Tamura C, Sanbo M, Hara H, Kato-Itoh M, Sato H, Kobayashi T, Nakauchi H, Hochi S^✝.
    Derivation of embryonic stem cell lines from parthenogenetically developing rat blastocysts.
    Stem Cells Dev. 2014 Jan 15;23(2):107-14. doi: 10.1089/scd.2013.0200. Epub 2013 Nov 7.
36. Matsunari H*, Nagashima H*^✝, Watanabe M, Umeyama K, Nakano K, Nagaya M, Kobayashi T, Yamaguchi T, Sumazaki R, Herzenberg LA, Nakauchi H^✝.
    Blastocyst complementation generates exogenic pancreas in vivo in apancreatic cloned pigs.
    Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4557-62. doi: 10.1262/jrd.2014-006. Epub 2014 Apr 21.
37. Hirabayashi M^✝, Tamura C, Sanbo M, Kato-Itoh M, Kobayashi T, Nakauchi H, Hochi S.
    A retrospective analysis of germline competence in rat embryonic stem cell lines.
    Transgenic Res. 2013 Apr;22(2):411-6. doi: 10.1007/s11248-012-9638-7. Epub 2012 Aug 9.
38. Yamaguchi T, Hamanaka S, Kamiya A, Okabe M, Kawarai M, Wakiyama Y, Umino A, Hayama T, Sato H, Lee YS, Kato-Itoh M, Masaki H, Kobayashi T, Yamazaki S, Nakauchi H^✝.
    Development of an all-in-one inducible lentiviral vector for gene specific analysis of reprogramming.
    PLoS One. 2012;7(7):e41007. doi: 10.1371/journal.pone.0041007. Epub 2012 Jul 18.
39. Kobayashi T, Kato-Itoh M, Yamaguchi T, Tamura C, Sanbo M, Hirabayashi M, Nakauchi H^✝.
    Identification of rat Rosa26 locus enables generation of knock-in rat lines ubiquitously expressing tdTomato.
    Stem Cells Dev. 2012 Nov 1;21(16):2981-6. doi: 10.1089/scd.2012.0065. Epub 2012 Jun 11.
40. Usui J*, Kobayashi T*, Yamaguchi T, Knisely AS, Nishinakamura R, Nakauchi H^✝.
    Generation of kidney from pluripotent stem cells via blastocyst complementation.
    Am J Pathol. 2012 Jun;180(6):2417-26. doi: 10.1016/j.ajpath.2012.03.007. Epub 2012 Apr 14.
41. Hirabayashi M, Tamura C, Sanbo M, Goto T, Kato-Itoh M, Kobayashi T, Nakauchi H, Hochi S^✝.
    Ability of tetraploid rat blastocysts to support fetal development after complementation with embryonic stem cells.
    Mol Reprod Dev. 2012 Jun;79(6):402-12. doi: 10.1002/mrd.22043. Epub 2012 May 4.
42. Hamanaka S, Yamaguchi T, Kobayashi T, Kato-Itoh M, Yamazaki S, Sato H, Umino A, Wakiyama Y, Arai M, Sanbo M, Hirabayashi M, Nakauchi H^✝.
    Generation of germline-competent rat induced pluripotent stem cells.
    PLoS One. 2011;6(7):e22008. doi: 10.1371/journal.pone.0022008. Epub 2011 Jul 15.
43. Nishimura K, Sano M, Ohtaka M, Furuta B, Umemura Y, Nakajima Y, Ikehara Y, Kobayashi T, Segawa H, Takayasu S, Sato H, Motomura K, Uchida E, Kanayasu-Toyoda T, Asashima M, Nakauchi H, Yamaguchi T, Nakanishi M^✝.
    Development of defective and persistent sendai virus vector: a unique gene delivery/expression system ideal for cell reprogramming.
    J Biol Chem. 2011 Feb 11;286(6):4760-71. doi: 10.1074/jbc.M110.183780. Epub 2010 Dec 7.
44. Kobayashi T, Yamaguchi T, Hamanaka S, Kato-Itoh M, Yamazaki Y, Ibata M, Sato H, Lee YS, Usui J, Knisely AS, Hirabayashi M, Nakauchi H^✝. 
    Generation of rat pancreas in mouse by interspecific blastocyst injection of pluripotent stem cells.
    Cell. 2010 Sep 3;142(5):787-99. doi: 10.1016/j.cell.2010.07.039.
    [Preview https://www.cell.com/fulltext/S0092-8674(10)00952-9]
45. Hirabayashi M, Kato M, Sanbo M, Kobayashi T, Hochi S, Nakauchi H^✝.
    Rat transgenesis via embryonic stem cells electroporated with the Kusabira-orange gene.
    Mol Reprod Dev. 2010 Jun;77(6):474. doi: 10.1002/mrd.21181.
46. Hirabayashi M, Kato M, Kobayashi T, Sanbo M, Yagi T, Hochi S, Nakauchi H^✝.
    Establishment of rat embryonic stem cell lines that can participate into germline chimerae at high efficiency.
    Mol Reprod Dev. 2010 Feb;77(2):94. doi: 10.1002/mrd.21123.
47. Okabe M, Otsu M*, Ahn DH, Kobayashi T, Morita Y, Wakiyama Y, Onodera M, Eto K, Ema H, Nakauchi H^✝.
    Definitive proof for direct reprogramming of hematopoietic cells to pluripotency.　
    Blood. 2009 Aug 27;114(9):1764-7. doi: 10.1182/blood-2009-02-203695. Epub 2009 Jun 29.
48. Eto K*^✝, Nishikii H*, Ogaeri T*, Suetsugu S, Kamiya A, Kobayashi T, Yamazaki D, Oda A, Takenawa T, Nakauchi H^✝.
    The WAVE2/Abi1 complex differentially regulates megakaryocyte development and spreading: implications for platelet biogenesis and spreading machinery.
    Blood. 2007 Nov 15;110(10):3637-47. doi: 10.1182/blood-2007-04-085860. Epub 2007 Jul 30.
49. Kobayashi T, Amemiya K, Takeuchi K, Tsujioka T, Tominaga K, Hirabayashi M, Ishikawa H, Fukui Y, Hochi S^✝.
    Contribution of spermatozoal centrosomes to microtubule-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis).
    Zygote. 2006 Feb;14(1):45-51. doi: 10.1017/S0967199406003522.
50. Iwanami Y, Kobayashi T, Kato M, Hirabayashi M, Hochi S^✝.
    Characteristics of rat round spermatids differentiated from spermatogonial cells during co-culture with Sertoli cells, assessed by flow cytometry, microinsemination and RT-PCR.,
    Theriogenology. 2006 Jan 20;65(2):288-98. doi: 10.1016/j.theriogenology.2005.04.025. Epub 2005 Jun 17.
51. Amemiya K, Iwanami Y, Kobayashi T, Terao T, Fukui Y, Ishikawa H, Ohsumi S, Hirabayashi M, Hochi S^✝. 
    Acquirement of oocyte-activating factor in Antarctic minke whale (Balaenoptera bonaerensis) spermatogenic cells, assessed by meiosis resumption of microinseminated mouse oocytes.
    Journal of Mammalian Ova Research. 2004 21(3):149-156. doi: 10.1274/jmor.21.149

Review

1. Alberio R^✝, Kobayashi T, Surani MA^✝.
   Conserved features of non-primate bilaminar disc embryos and the germline.
   Stem Cell Reports. 2021 May 11;16(5):1078-1092. doi: 10.1016/j.stemcr.2021.03.011.
2. Kobayashi T, Surani MA^✝.
   On the origin of the human germline.
   Development. 2018 Jul 23;145(16). pii: dev150433. doi: 10.1242/dev.150433.
3. Tang WW, Kobayashi T, Irie N, Dietmann S, Surani MA^✝.
   Specification and epigenetic programming of the human germ line.
   Nat Rev Genet. 2016 Oct;17(10):585-600. doi: 10.1038/nrg.2016.88.
4. Rashid T, Kobayashi T, Nakauchi H^✝.
   Revisiting the Flight of Icarus: Making Human Organs from PSCs with Large Animal Chimeras.
   Cell Stem Cell. 2014 Oct 2;15(4):406-9. doi: 10.1016/j.stem.2014.09.013.